CRISPR/Cas9 Optimization Using Electronic Genome Mapping: Potential Role for Studying Human Genetic Disease
Lindsay Schneider Opsahl, Wentao Huang, Syndi Koltz, Kaylee L. Mathews, John F. Thompson
This study uses electronic genome mapping to analyze how gRNA mismatches affect CRISPR/Cas9 binding specificity across the human genome, revealing key regions for on- and off-target activity.
bioRxiv · Sep 17, 2026 · PreprintGenomics