Conformation-specific Antibody Deciphers K27-linked Ubiquitination in Chaperone-Mediated Proteostasis
Chunyan Han, Yicheng Weng, Qingyun Zheng, Qian Qu, Satchal K. Erramilli, Zhen Chao Su, Yujuan Duan, Yunxi Han, Xiaoguo Zhai, Jingxian Li, Anthony A. Kossiakoff, Man Pan, Minglei Zhao, Lei Liu, Yuanyuan Yu
This paper develops a conformation-specific antibody, K27-IgG, to detect K27-linked polyubiquitin and identifies DNAJB1 as a reader and UBE2Q1 as a writer for this ubiquitin chain type.
bioRxiv · Dec 18, 2025 · PreprintBiochemistry