Determine Interaction Affinity Changes of the SUMO E1 Activating Enzymes During SUMO Activation using Quantitative FRET technology
Ling Jiang, Yiran Tao, Xin Wen, Jiayu Liao
The paper determines the interaction affinity and kinetics of the Aos1-Uba2 heterodimer during SUMO activation using qFRET and SPR, showing increased stability with SUMO and ATP binding.
bioRxiv · Jul 30, 2025 · PreprintBioengineering