Jiayu Liao

The paper determines the interaction affinity and kinetics of the Aos1-Uba2 heterodimer during SUMO activation using qFRET and SPR, showing increased stability with SUMO and ATP binding.

This paper develops a high-throughput qFRET method to determine protein interaction affinity without purification, revealing a two-enzyme co-recognition mechanism for SUMOylation substrates.

The paper identifies mitochondrial RNAs (mtRNAs) as chromatin-associated RNAs (caRNAs) that regulate nuclear transcription in response to stress and disease.