RoCi - A Single Step Multi-Copy Integration System Based on Rolling-Circle Replication
Martzel Antsotegi-Uskola, Vasil D’Ambrosio, Brian King Himm Mo, Zofia Dorota Jarczynska, Katherina García Vanegas, Martí Morera-Gómez, Xinhui Wang, Thomas Ostenfeld Larsen, Jean‐Marie Mouillon, Uffe Hasbro Mortensen
The paper introduces RoCi, a method for integrating multiple directly repeated gene-expression cassettes into a defined genomic locus in fungi, resulting in stable and high-yield production of metabolites and enzymes.
Nucleic Acids Research · Dec 17, 2025Peer-reviewedSynthetic Biology