Zofia Dorota Jarczynska

The paper introduces RoCi, a method for integrating multiple directly repeated gene-expression cassettes into a defined genomic locus in fungi, resulting in stable and high-yield production of metabolites and enzymes.

This study develops a CRISPR-Cas9 toolbox for efficient genetic engineering of Komagataella phaffii, enabling marker-free gene integration and high-efficiency mutagenesis.

This study combines ALE and WT variants to explore sequence solutions for industrial tolerance in E. coli, finding that WT variants can provide greater benefits and help identify feasible genome design variables.