Uffe Hasbro Mortensen

The paper introduces RoCi, a method for integrating multiple directly repeated gene-expression cassettes into a defined genomic locus in fungi, resulting in stable and high-yield production of metabolites and enzymes.

The paper uses genome-wide host-pathway interaction screens to identify metabolic targets for optimizing cis-cis-muconic acid production in yeast.

This study develops a CRISPR-Cas9 toolbox for efficient genetic engineering of Komagataella phaffii, enabling marker-free gene integration and high-efficiency mutagenesis.

CRI-SPA transfers genetic features between yeast strains to map sequence to function, demonstrating its ability to transfer genes for betaxanthin production across a yeast knock-out collection.

This study optimizes benzylglucosinolate production in yeast by comparing plasmid and genome integration methods and enhancing key enzymes in the biosynthetic pathway.