Growth-coupled continuous directed evolution by MutaT7 enables efficient and automated enzyme engineering
Yijie Deng, Kai Etheridge, Xinping Ran, Hannah E. Maurais, Rahul Sarpeshkar
This paper introduces a continuous directed evolution method called GCCDE that automates enzyme engineering by linking enzyme activity to bacterial growth, demonstrating its effectiveness in improving CelB's low-temperature activity.
Applied and Environmental Microbiology · Mar 27, 2025Peer-reviewedBioengineering