Author

Rahul Sarpeshkar

Dartmouth CollegeORCID 0000-0002-5975-0004Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Bioengineering· 1 Microbiology· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:cell type annotation· 1 Approach:deep learning· 1 Organism:E. coli· 1 Research Focus:protein folding & dynamics· 1 Assay:proteomics· 1 Approach:statistical model· 1

Frequent Co-Authors

Hannah E. MauraisiD· 2 shared Kai Etheridge· 2 shared Yijie DengiD· 2 shared Areen BanerjeeiD· 1 shared Daniel SchultziD· 1 shared Douglas Raymond BeahmiD· 1 shared Jaewook KimiD· 1 shared Ji ZengiD· 1 shared

Activity by Year

2018-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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2 shared papersMicrobiology

Hannah E. MauraisORCID

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3 papers

Recent·Most cited

Antibiotic exposure dynamically generates a substantial number of heterogeneous persisters along a spectrum of tolerance

Yijie Deng, Douglas Raymond Beahm, Hannah E. Maurais, Kai Etheridge, Daniel Schultz, Rahul Sarpeshkar

This study quantifies pre-existing and drug-induced persisters using a framework combining kinetic modeling and SDTK assays, revealing antibiotic-specific persistence dynamics and a tolerance spectrum.

bioRxiv · Jan 31, 2026 · PreprintMicrobiology

Growth-coupled continuous directed evolution by MutaT7 enables efficient and automated enzyme engineering

Yijie Deng, Kai Etheridge, Xinping Ran, Hannah E. Maurais, Rahul Sarpeshkar

This paper introduces a continuous directed evolution method called GCCDE that automates enzyme engineering by linking enzyme activity to bacterial growth, demonstrating its effectiveness in improving CelB's low-temperature activity.

Applied and Environmental Microbiology · Mar 27, 2025Peer-reviewedBioengineering

A Synthetic Microbial Operational Amplifier

Ji Zeng, Jonathan Jin Yuan. Teo, Areen Banerjee, Timothy W. Chapman, Jaewook Kim, Rahul Sarpeshkar

The paper describes a synthetic bio-molecular operational amplifier using 5 proteins to enable robust, precise, and programmable homeostasis and regulation in living cells.

ACS Synthetic Biology · Aug 28, 2018Peer-reviewedSynthetic Biology