Yijie Deng

This study quantifies pre-existing and drug-induced persisters using a framework combining kinetic modeling and SDTK assays, revealing antibiotic-specific persistence dynamics and a tolerance spectrum.

This paper introduces a continuous directed evolution method called GCCDE that automates enzyme engineering by linking enzyme activity to bacterial growth, demonstrating its effectiveness in improving CelB's low-temperature activity.

This study shows that Pseudomonas aeruginosa resistance to aminoglycosides is heterogeneous, with mexZ mutations improving survival by bypassing slow MexXY activation, and introduces a 'Response Dynamics' assay to measure resistance in fluctuating drug environments.