A single mutation attenuates both the transcription termination and RNA-dependent RNA polymerase activity of T7 RNA polymerase
Hui Ming Wu, Ting Wei, Bingbing Yu, Rui Cheng, Fengtao Huang, Xuelin Lu, Yan Yan, Xionglue Wang, Chenli Liu, Bin Zhu
The paper identifies a single mutation, S43Y, that reduces transcription termination and RNA-dependent RNA polymerase activity in T7 RNA polymerase.
RNA Biology · Jul 27, 2021Peer-reviewedMolecular Biology