Bin Zhu

This study identifies DNA-terminus-initiated transcription by T7 RNA polymerase as the main source of dsRNA byproducts and shows that a C-helix mutant reduces dsRNA production and improves mRNA quality.

This study shows SARS-CoV-2 nsp15 preferentially cleaves AU-rich dsRNA via nicking, supporting its role in evading host dsRNA sensors by degrading viral replication intermediates.

The paper identifies a single mutation, S43Y, that reduces transcription termination and RNA-dependent RNA polymerase activity in T7 RNA polymerase.

This paper characterizes a psychrophilic phage RNA polymerase that improves RNA purity and stability in vitro transcription by reducing dsRNA by-products and RNA degradation.