Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
O. Israeli, A. Beth-Din, N. Paran, D. Stein, S. Lazar, S. Weiss, E. Milrot, Y. Atiya-Nasagi, S. Yitzhaki, O. Laskar, O. Schuster
The paper compares RT-qPCR efficacy with and without RNA extraction for SARS-CoV-2 detection, finding that extraction is advantageous but a direct no-buffer approach can identify up to 70% of clinical specimens.
bioRxiv · Jun 10, 2020Microbiology