S. Weiss

P-BODY AND CYTOSKELETON REMODELING BY ORTHOHANTAVIRUSES.

This study uses advanced imaging to analyze how orthohantaviruses alter P-bodies and the cytoskeleton during infection, revealing spatial correlations and degradation patterns of viral RNAs.

Towards optical measurements of membrane potential values in Bacillus subtilis using fluorescence lifetime

This paper introduces a fluorescence lifetime-based method to measure membrane potential in Bacillus subtilis, achieving single-cell resolution and quantification of MP changes under chemical perturbations.

Exploiting a metabolic vulnerability in brain tumour stem cells using a brain-penetrant drug with safe profile

This study shows that mubritinib targets brain tumour stem cells by inhibiting complex I of the electron transport chain, delays glioblastoma tumourigenesis, and is safe in mice.

Doxycycline Levofloxacin or Moxifloxacin are superior to ciprofloxacin in treating anthrax meningitis in rabbits

This study compares the efficacy of Doxycycline, Levofloxacin, and Moxifloxacin to Ciprofloxacin in treating anthrax meningitis in rabbits, finding the former more effective.

Tripartite interactions of PKA catalytic subunit and C-terminal domains of cardiac Ca2+ channel modulate its β-adrenergic regulation

This study identifies direct interactions between PKA catalytic subunit and C-terminal domains of cardiac CaV1.2 channels, revealing their role in modulating β-adrenergic regulation.

Structural basis of transcription: RNA Polymerase II substrate binding and metal coordination at 3.0 A using a free-electron laser

This paper presents the structure of ATP-bound RNA Polymerase II at 3.0 Å resolution using a free-electron laser, revealing new insights into substrate binding and metal coordination in the active site.

Autophagy in maternal tissues contributes to Arabidopsis thaliana seed development.

This study investigates the role of autophagy in maternal tissues during Arabidopsis thaliana seed development, showing its impact on nutrient remobilization and seed coat development.

Adult thymus-derived cMaf+ RORγt+ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

This study evaluates the reliability and accuracy of single-molecule FRET for measuring protein structural dynamics and distances, finding high precision and accuracy with specific uncertainty limits.

Monkeypox DNA correlates with virus infectivity in clinical samples

The paper correlates Monkeypox DNA content with virus infectivity in clinical samples and defines a PCR threshold for non-infectious virus.

Ecological Dynamics Imposes Fundamental Challenges in Microbial Source Tracking

This study shows that existing microbial source tracking solvers fail to solve the MST problem when ecological dynamics, such as microbial interactions and priority effects, are present.

Parsing the role of NSP1 in SARS-CoV-2 infection

This study investigates the role of SARS-CoV-2 nsp1 in host shutoff and immune evasion, finding it inhibits translation and induces mRNA degradation, and is critical for counteracting the IFN response.

Alternate response of Bacillus anthracis atxA and acpA to serum, HCO3- and CO2

This study investigates how Bacillus anthracis regulates toxin and capsule production in response to serum, HCO3-, and CO2 using a modified medium system.

In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera

This study demonstrates the use of a time-gated SPAD camera for in vitro and in vivo NIR fluorescence lifetime imaging, showing its effectiveness in monitoring molecular events in tumor xenografts.

Electrically Controlling and Optically Observing the Membrane Potential of Supported Lipid Bilayers

This paper describes a platform for supported lipid bilayers that can be electrically controlled and optically probed to study membrane polarization dynamics.

PySOFI: an open source Python package for SOFI

The paper introduces PySOFI, an open-source Python package for SOFI analysis, enabling flexible algorithm inspection, testing, modification, and extension.

Long-chain lipids facilitate insertion of large nanoparticles into membranes of small unilamellar vesicles

The paper investigates how long-chain lipids enhance nanoparticle insertion into small unilamellar vesicles by reducing membrane disruption.

Nonclinical Safety and Immunogenicity of an rVSV-ΔG-SARS-CoV-2-S vaccine in mice, hamsters, rabbits and pigs

This study evaluates the safety, immunogenicity, and efficacy of an rVSV-ΔG-SARS-CoV-2-S vaccine in multiple animal species, finding no adverse effects and a robust immune response.

Rapid Ensemble Measurement of Protein Diffusion, Probe Blinking and Photobleaching Dynamics in the Complex Cellular Space

The paper introduces a fluorescence fluctuation image correlation analysis method to measure diffusion, blinking, and photobleaching dynamics in cells, validated on simulations and live cells.

Image quantification technique reveals novel lung cancer cytoskeletal phenotype with partial EMT signature

The paper identifies an intermediate EMT state with a specific cytoskeletal signature using image quantification tools and tracks EMT progression and drug responses.

Monitoring group activity of hamsters and mice as a novel tool to evaluate COVID-19 progression, convalescence and rVSV-ΔG-spike vaccination efficacy

This study uses group activity monitoring in hamsters and mice to assess SARS-CoV-2 infection, convalescence, and vaccine efficacy.

Point-localized and site-specific membrane potential optical recording by engineered single fluorescent nanodiscs

The paper develops a FRET-based voltage sensing method using fluorescent nanodiscs to record membrane potentials at the single-nanodisc level in HEK293 and cortical neuron cells.

Optical Probing of Local Membrane Potential with Fluorescent Polystyrene Beads

The paper introduces a new optical voltage-imaging technique using fluorescent polystyrene beads and Dipicrylamine to measure membrane potential with single-particle resolution.

Multi-parameter photon-by-photon hidden Markov modeling

This paper introduces multi-parameter H2MM to distinguish FRET dynamics from photophysical transitions in smFRET data, improving the analysis of biomolecular conformational changes.

The neutralization potency of anti-SARS-CoV-2 therapeutic human monoclonal antibodies is retained against novel viral variants

This study evaluates the neutralization potency of four SARS-CoV-2 mAbs against spike mutations and two variants of concern, finding that MD65 and BL6 retain effectiveness against B.1.1.7 and B.1.351.

Therapeutic antibodies, targeting the SARS-CoV-2 spike N-terminal domain, protect lethally infected K18-hACE2 mice

This study identifies and characterizes neutralizing antibodies targeting the SARS-CoV-2 spike N-terminal domain, showing their protective efficacy in mice.

Reconstitution of β-adrenergic regulation of CaV1.2: Rad-dependent and Rad-independent protein kinase A mechanisms.

This study reconstitutes the β-adrenergic regulation of CaV1.2 channels in a heterologous system, identifying Rad-dependent and Rad-independent PKA mechanisms.

The coding capacity of SARS-CoV-2

This study maps the full coding capacity of SARS-CoV-2 using ribosome profiling, identifying 23 novel translated ORFs and revealing viral translation dominance over host translation.

A single dose of recombinant VSV-{triangleup}G-spike vaccine provides protection against SARS-CoV-2 challenge

This study develops a recombinant VSV-ΔG-spike vaccine that provides single-dose protection against SARS-CoV-2 in hamsters by inducing neutralizing antibodies and preventing lung pathology.

Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction

The paper compares RT-qPCR efficacy with and without RNA extraction for SARS-CoV-2 detection, finding that extraction is advantageous but a direct no-buffer approach can identify up to 70% of clinical specimens.