Y. Nagira

DNA-free and genotype-independent CRISPR/Cas9 system in soybean

This paper develops a DNA-free CRISPR/Cas9 system in soybean using iPB-RNP method, achieving genome editing in shoot apical meristems without tissue culture, resulting in 0.4-4.6% mutation transmission to next generation.

In planta genome editing with CRISPR/Cas9 ribonucleoproteins

This study uses CRISPR/Cas9 ribonucleoproteins delivered via particle bombardment to edit the wheat genome, resulting in a 10% reduction in culm length without yield loss.