Mining of natural diversity enables efficient and expressible peptide asparaginyl ligases
Wenyu Du, Shi Qi, 甄明明, Miao Liao, Yang Zhao, Jiahui Ma, Yining Hao, Jiang Hui, Gengyu Lu, Chong Wai Liew, Niying Chua, Huachao Chen, Guangwan Hu, Xinya Hemu
The paper discovers new peptide asparaginyl ligases (PALs) from Viola species, identifies a highly efficient variant, and develops a structure-based engineering strategy to improve expression.
Nature Communications · Apr 27, 2026Peer-reviewedBiochemistry