Harnessing anti-CRISPR to suppress, subtract, and segregate Cas9 activity for precision CRISPR in Drosophila
Yifan Shen, Michael Sheen, Ann T. Yeung, Xinchen Chen, Bei Wang, Zixian Huang, Claire A. Ho, Zachary I. Lakkis, Chun Han
This paper develops a toolkit using AcrIIA4 to suppress, subtract, and segregate Cas9 activity in Drosophila for more precise CRISPR applications.
bioRxiv · Dec 9, 2025 · PreprintGenetics