Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment
Seung-Hun Kang, Wi-jae Lee, Ju-Hyun An, Jong‐Hee Lee, Young-Hyun Kim, Hanseop Kim, Yeounsun Oh, Young‐Ho Park, Yeung Bae Jin, Bong‐Hyun Jun, Junho K. Hur, Sun‐Uk Kim, Seung Hwan Lee
The paper develops a method to detect CRISPR off-target mutations with high sensitivity using predicted off-target-specific amplification, showing significantly improved detection rates compared to existing methods.
Nature Communications · Jul 17, 2020Peer-reviewedMolecular Biology