Seung Hwan Lee

This study optimizes twin prime editing components to improve genome editing efficiency and accuracy for therapeutic applications in spinocerebellar ataxia type 3.

This paper introduces a CRISPR-Cas9 editing strategy using near-complementary sgRNAs to specifically target single-nucleotide mutations without relying on PAM sequences, demonstrating its effectiveness on a cancer-related mutation.

The paper was withdrawn due to significant content changes, and the authors do not wish it to be cited.

The paper develops a method to detect CRISPR off-target mutations with high sensitivity using predicted off-target-specific amplification, showing significantly improved detection rates compared to existing methods.

The paper demonstrates that NgAgo from Natronobacterium gregoryi cleaves RNA instead of DNA, functioning as a DNA-guided endoribonuclease.