Rahmi RL Lale

The paper introduces an integrated microfluidic workflow for high-throughput cell-free transcription using double emulsion picoreactors, enabling quantitative screening of regulatory sequences without specialized sorting equipment.

The paper maps sigma54 DNA binding sequences in Pseudomonas putida using a data-driven approach combining DNA libraries, RNA aptamers, and deep sequencing, revealing 64,966 distinct motifs.

This paper develops a genetic switch for Lactiplantibacillus plantarum using a bacteriophage promoter and cumate inducer, and shows that encapsulation in alginate reduces leaky expression and improves switch performance.

This study develops and validates artificial 5' regulatory sequences for enhancing thermostable protein expression in Thermus thermophilus.

This paper introduces a DNA design platform using Transformer models to predict cross-species DNA functionality, enabling context-sensitive and host-specific gene expression optimization.

The paper identifies a short fluorescent protein isoform in mCherry that causes background fluorescence and proposes a modified version to improve reporter function.

The paper designs a novel dual UTR for synthetic biology that separately enhances transcription or translation in E. coli and P. putida.