Shrikrishnan Sankaran

The paper develops an engineered living material that uses NIR light to release a pro-angiogenic peptide, promoting capillary network formation in endothelial cultures.

This study investigates how mechanical confinement in hydrogels affects growth, metabolism, and gene expression in gram-negative and gram-positive probiotic bacteria.

This study develops PEARL, an engineered living material using encapsulated recombinant lactobacilli in alginate beads for controlled protein release over 14 days.

This paper develops a genetic switch for Lactiplantibacillus plantarum using a bacteriophage promoter and cumate inducer, and shows that encapsulation in alginate reduces leaky expression and improves switch performance.

The paper develops a 96-well plate-based screening method to evaluate ELM growth and host compatibility in vitro.

The paper develops a thermo-amplifier circuit in probiotic E. coli to control the release of darobactin, a peptide antibiotic, at 40°C while maintaining low leakiness at 37°C.

This study shows that genetically programmed mechanical properties of bacterial engineered living materials modulate mechanosignaling in mammalian cells.

The paper identifies a high-performance phage-derived promoter/repressor system for Lactobacillus engineering, achieving high expression and strong repression in L. plantarum WCFS1.

This paper describes two genetic modules in Lactiplantibacillus plantarum that enable high-level gene expression without antibiotics, bacteriocins, or genomic manipulations.

This paper develops a direct cloning method using Gibson assembly and PCR to efficiently transform Lactiplantibacillus plantarum WCSF1 with genes incompatible with E. coli.