Author

Johann Engelhardt

Max Planck Institute for Medical ResearchORCID 0000-0001-5487-8933Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:benchmarking· 1 Research Focus:cell signaling & trafficking· 1 Assay:fluorescence / live-cell microscopy· 1 Assay:imaging· 1 Approach:microscopy & imaging analysis· 1 Organism:mouse· 1

Frequent Co-Authors

Stefan W. HelliD· 2 shared Elisa D’EsteiD· 1 shared Jan Otto WirthiD· 1 shared Jasmine HubrichiD· 1 shared Jonas M. SchleskeiD· 1 shared Katarina B. ChapmaniD· 1 shared Roman SchmidtiD· 1 shared

Activity by Year

2024-2026 · peak 1/yr

Co-Author Collaboration Network

7 top collaborators · 13 mutual co-author links

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2 shared papersBiophysics

Stefan W. HellORCID

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2 papers

Recent·Most cited

Localization accuracy in single-molecule fluorescence microscopy

Katarina B. Chapman, Roman Schmidt, Johann Engelhardt, Stefan W. Hell

This study compares the accuracy of two single-molecule localization methods in microscopy, finding that MINFLUX significantly outperforms camera-based methods.

bioRxiv · Oct 16, 2026 · PreprintBiophysics

MINFLUX Reveals Dynein Stepping in Live Neurons

Jonas M. Schleske, Jasmine Hubrich, Jan Otto Wirth, Elisa D’Este, Johann Engelhardt, Stefan W. Hell

This study uses MINFLUX to track dynein movement in live neurons, revealing 8 nm steps and rapid direction reversals, suggesting a single ATP-dependent stepping mechanism.

Proceedings of the National Academy of Sciences · Sep 10, 2024Peer-reviewedBiophysics