James Nathan Cobley

CysNet infers cysteine redox proteoforms from mass spectrometry data, resolving oxiform states and quantifying their abundance in human stem cells.

ReCap enables redox proteomics by preserving redox states and scaling oxidation to protein copy numbers, revealing sparse and quantitatively concentrated cysteine oxidation signals.

The paper introduces Cleland immunoblotting, a method to detect cysteine redox proteoforms by reversing PEG-payload mobility shifts, revealing previously undetectable proteoforms in Xenopus oocytes.

The paper computationally analyzes the human proteome to estimate the vast number of cysteine redox proteoforms and derives theoretical limits based on protein copy numbers and thermodynamic equilibrium.