Chuanfeng Wu

This study compares CRISPR/HDR and lentiviral transduction effects on HSPC engraftment and clonal dynamics in rhesus macaques, finding HDR-edited cells decline rapidly while lentiviral cells persist longer.

barcodetrackR provides tools for analyzing clonal tracking data to study lineage reconstitution and clonal dynamics in gene and cell therapy studies.

This paper reports a case of clonal hematopoiesis in a rhesus macaque following lentiviral transduction of HSPC with a strong retroviral promoter, leading to gene overexpression and aberrant splicing.