Christopher Ashwood

GlyComboCLI assigns glycan compositions to mass spectrometry data through a command-line interface, supporting diverse parameters and integration into FAIR workflows.

The paper develops an enrichment-free glycoproteomics method using mass defect-driven glycopeptide classification to identify sex differences in murine plasma fucosylation.

This paper develops a matrix-matched calibration curve for quantifying Neu5Gc-bearing N-glycans across multiple sites, showing LC-MS outperforms DMB-LC-FLR in dynamic range and sensitivity.

This paper introduces GlycoRTMS, a real-time mass spectrometry method that improves glycosylation detection by using glycan composition context to guide fragmentation and prioritization.

The paper introduces N-glycopedia, a resource of N-glycan standards and analytical methods for structural analysis, enabling more accurate glycomics research.

This study develops a native PGC-LC-MS workflow to detect O-acetylated sialylated N-glycans in mammalian sera, revealing species-specific patterns of modification.

This paper introduces a supercharging method using HFIP and butylamine to improve mass spectrometric analysis of oxidatively released O-glycans, enabling accurate structural assignment and discrimination of isomeric compositions.

This study identifies RbmB as a polysaccharide lyase that cleaves Vibrio cholerae VPS, contributing to biofilm dispersal and suggesting its potential as an antibacterial enzyme.

The paper describes an e-infrastructure for recording glycomic mass spectrometric data in compliance with MIRAGE reporting guidelines, enabling standardized data submission to a public repository.