Systematic Characterization of Optical Aberrations Reveals Cryo-FLM Localization Fidelity
Hongjia Li, Lauren Ann Metskas, Fang Ping Huang
This study systematically analyzes optical aberrations in cryo-FLM, showing they cause localization errors up to 300 nm axially and introduces models to reduce errors to under 10 nm.
bioRxiv · Jul 25, 2025 · PreprintBioinformatics