Benjamin R. Jack

This study shows that transcript degradation and codon usage regulate gene expression in bacteriophage T7 through a dynamic interplay between transcription, translation, and transcript degradation.

Pinetree simulates gene expression with codon-specific translation rates and provides a Python interface for genome-based simulations.

The paper investigates how codon deoptimization in bacteriophage T7 reduces protein expression and viral fitness by halving translation of key genes.