Multiparameter optimization extends the lifetime of cell-free protein synthesis in a high-throughput format
Eray Ulaş Bozkurt, Baptiste Zanchet, Pablo I. Nikel, Daniel C. Volke
This study optimizes DNA template design, reaction composition, and lysate genotype to extend CFPS reaction lifetime in a high-throughput format, achieving >14 h productivity and high deGFP yield.