This paper introduces a high-accuracy CRISPR array assembly strategy that enables efficient assembly of multiple crRNAs in a single reaction, improving the practicality of multiplex CRISPR applications.

Molecular Therapy — Nucleic Acids · Dec 12, 2024Peer-reviewedSynthetic Biology

PKHD1L1, A Gene Involved in the Stereocilia Coat, Causes Autosomal Recessive Nonsyndromic Hearing Loss

Shelby E. Redfield, Pedro De‐la‐Torre, Mina Zamani, Hanjun Wang, Hina Khan, Tyler Morris, Gholamreza Shariati, Majid Karimi, Margaret A. Kenna, Go Hun Seo, Hongen Xu, Wei Lu, Sadaf Naz, Hamid Galehdari, Artur A. Indzhykulian, , Barbara Vona

This study identifies PKHD1L1 as a cause of autosomal recessive nonsyndromic hearing loss through exome sequencing and functional analysis in human families and animal models.

Human Genetics · Mar 1, 2024Peer-reviewedGenetic & Genomic Medicine

Defining the Sensitivity Landscape of 74,389 EGFR Variants to Tyrosine Kinase Inhibitors

Lei An, Shuqing Chen, Guangyao Wu, Chang Liu, Zhenxing Wang, Chunli Wang, Zeyuan Shi, Chenguang Niu, Xiaodong Li, Wenxue Tang, Hongen Xu, Yueqiang Wang

The paper systematically studies the sensitivity of 74,389 EGFR variants to five tyrosine kinase inhibitors using cytotoxicity assays and presents a comprehensive reference for treatment responses.

bioRxiv · Jul 18, 2021 · PreprintGenetics

SARS-CoV-2 infecting the inner ear results in potential hearing damage at the early stage or prognosis of COVID-19 in rodents

Xia Xue, Yongan Tian, Mingsan Miao, Jianyao Wang, Wenxue Tang, Yaohe Wang, Jianbo Liu, Hongen Xu, Jinxin Miao

The paper investigates ACE2 and TMPRSS2 expression in the inner ear, brain, and muscle of mice and hamsters to assess SARS-CoV-2 infection potential and its link to sensorineural hearing loss in COVID-19.

bioRxiv · Dec 23, 2020 · PreprintBioinformatics