Author

Nadja A. Simeth

Multiscale BioimagingORCID 0000-0001-8130-883XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:imaging· 2 Research Focus:cell type annotation· 1 Research Focus:clustering· 1 Assay:fluorescence / live-cell microscopy· 1 Approach:microscopy & imaging analysis· 1 Research Focus:spatial domain detection· 1

Frequent Co-Authors

Elena R. CotroneoiD· 2 shared Felipe OpazoiD· 2 shared Nikolaos MougiosiD· 2 shared Roman TsukanoviD· 2 shared Henrike Koerner· 1 shared Johannes BroichhageniD· 1 shared Jonas SetzkeiD· 1 shared Jörg EnderleiniD· 1 shared

Activity by Year

2024-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 40 mutual co-author links

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2 shared papersCell Biology

Elena R. CotroneoORCID

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2 papers

Recent·Most cited

Cell BiologybioRxivPreprintMar 2, 2026

Nanobodies equipped with HaloTag variants enable rapid and straightforward one-step immunofluorescence lifetime multiplexing

László Albert, Samrat Basak, Henrike Koerner…·Felipe Opazo

The paper introduces nanobody-HaloTag constructs for multiplexed immunofluorescence imaging, enabling up to eight targets with lifetime and spectral encoding in a single step.

DOI 10.64898/2026.03.02.709025

imagingfluorescence / live-cell microscopy

𝕏 PostBluesky

NeuroscienceNature CommunicationsPeer-reviewedOct 9, 2024

NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals

Nikolaos Mougios, Elena R. Cotroneo, Nils Imse…·Felipe Opazo

NanoPlex enables multiplex fluorescence microscopy using nanobodies with erasable signals, achieving up to 21 targets in 3D confocal imaging.

28 citationstop 3%

imagingexperimental assay

𝕏 PostBluesky