Nanobodies equipped with HaloTag variants enable rapid and straightforward one-step immunofluorescence lifetime multiplexing
László Albert, Samrat Basak, Henrike Koerner, Nazar Oleksiievets, Nikolaos Mougios, Elena R. Cotroneo, Michelle S. Frei, Jörg Enderlein, Johannes Broichhagen, Nadja A. Simeth, Roman Tsukanov, Felipe Opazo
The paper introduces nanobody-HaloTag constructs for multiplexed immunofluorescence imaging, enabling up to eight targets with lifetime and spectral encoding in a single step.
bioRxiv · Mar 2, 2026 · PreprintCell Biology