Felipe Opazo

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy.

This paper demonstrates that self-blinking dye JF635b improves nanobody performance in single-molecule localization microscopy, enabling reliable imaging across multiple modalities with high precision.

A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications.

This paper introduces sdAb(mNG), a high-affinity nanobody that enables versatile applications for mNG-tagged proteins, including imaging, immunoprecipitation, and in vivo manipulation.

Fatty acid binding protein-8 (FABP8/PMP2) reveals molecular heterogeneity of myelin sheaths in the human CNS

This paper identifies FABP8/PMP2 as a defining feature of human and old-world monkey CNS myelin, revealing molecular heterogeneity in myelin sheaths and its role in cholesterol enrichment.

Nanobodies equipped with HaloTag variants enable rapid and straightforward one-step immunofluorescence lifetime multiplexing

The paper introduces nanobody-HaloTag constructs for multiplexed immunofluorescence imaging, enabling up to eight targets with lifetime and spectral encoding in a single step.

A versatile microfluidics platform for enhanced multi-target super-resolution microscopy

This paper introduces a microfluidics platform for multiplexed super-resolution microscopy, demonstrating its use in imaging cancer and cardiac cells with high reproducibility.

Nanobinders for Synaptotagmin 1 enable the analysis of synapticvesicle dynamics in rodent and human models.

The paper introduces NbLumSyt1 nanobinders for analyzing synaptic vesicle dynamics in rodent and human models with improved precision and versatility.

Nanobodies against the myelin enzyme CNPase as tools for structural and functional studies

This paper develops nanobodies against CNPase, showing distinct epitopes and functional applications in imaging and as intrabodies.

NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals

NanoPlex enables multiplex fluorescence microscopy using nanobodies with erasable signals, achieving up to 21 targets in 3D confocal imaging.

Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT

This paper introduces MIET-PAINT, a method combining DNA-PAINT and Metal-Induced Energy Transfer to achieve nanometer-scale 3D imaging with high multiplexing capability.

Spatial proteomics in neurons at single-protein resolution

This paper introduces SUM-PAINT, a high-throughput imaging method for single-protein spatial resolution, and discovers a new synapse type through extensive multiprotein analysis.

Neuronal activity modulates the incorporation of newly translated PSD-95 into a robust structure as revealed by STED and MINFLUX

This study uses advanced imaging techniques to show how synaptic activity affects the incorporation of newly translated PSD-95 into synaptic clusters at near-molecular resolution.

A versatile Synaptotagmin-1 nanobody provides perturbation-free live synaptic imaging and low linkage-error in super-resolution microscopy

This paper introduces a nanobody that enables non-invasive live synaptic imaging and improves super-resolution microscopy with minimal physiological impact.

Simple and highly efficient detection of PSD95 using a nanobody and its re-combinant minibody derivative

This paper describes a nanobody and its minibody derivative for highly specific and efficient detection of PSD95 across multiple species.

Optogenetics and electron tomography for structure-function analysis of cochlear ribbon synapses

This study uses optogenetics and electron tomography to analyze the ultrastructure of cochlear inner hair cell ribbon synapses during stimulation, showing increased docked synaptic vesicles and no evidence of homotypic fusion.

A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol

The paper develops a fluorescent reporter (FluoReSyn) using a nanobody to detect human α-synuclein in cells and tissues, showing its presence in cerebrospinal fluid.

Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons

This study uses nanobodies to show that SNAP-25 and Syntaxin 1A have extra-synaptic populations in hippocampal neurons, which are not well detected by conventional antibodies.