Friedrich Preußer

This paper introduces FRC-QE, a 3D image quality metric for cleared organoids, showing it robustly captures clearing efficiency differences across protocols and microscopy modalities.

BigStitcher reconstructs high-resolution image datasets from cleared and expanded biological samples, compensating optical distortions for single-cell analysis.

The paper introduces pheno-seq, a method that links morphological features to gene expression in 3D cell cultures, showing its ability to identify morphology-specific genes and regulatory states not captured by scRNA-seq.