Stephen L. Hart

This study shows that K978C CFTR, which increases open probability, restores epithelial function more efficiently than wildtype CFTR in CF airway cells when expressed in the presence of CF sputum.

The paper introduces FIVER, a multispectral reporter for in vivo genome editing that detects NHEJ, HDR, and HITI with fluorescent read-outs, demonstrating its utility in primary cells, organoids, and in vivo models.