A membrane-impermeant nucleic acid dye converts bacteriophage plaque assays into a machine-readable format for automated counting
Alissa Wiwi, Jack H. Arnold, Darren W. Branch, Jesse L. Cahill
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Sandia National LaboratoriesORCID 0000-0001-8387-9273Unclaimed ORCID profile · follow to get notified of new preprints
5 papers
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Alissa Wiwi, Jack H. Arnold, Darren W. Branch, Jesse L. Cahill
Eric M. Small, Greyson Lasley, Emily M. Layton…·Jesse L. Cahill
The paper reconstructs the MS2 phage genome using cDNA and shows that the current NCBI reference genome does not produce infectious phage, highlighting the need to verify reference genome infectivity.
Emily M. Layton, Michael Bernauer, Laura D. Weinstock…·Jesse L. Cahill
This study evaluates a genome language model's ability to predict the effects of sequence changes in phages using experimental data, finding mixed success in distinguishing functional from nonfunctional mutations.
Jack Arnold, Isabella G. Romano, Devyn Del Curto…·Jesse L. Cahill
The paper identifies temperature-responsive integrases from extremophiles and demonstrates their use in creating temperature-switchable genome editing systems.
Devyn Del Curto, Brittany Humphrey, Greyson Lasley…·Jesse L. Cahill
This study re-examines pseudolysogeny in phage T3 and T7 under nutrient limitation, finding that both show impaired propagation, and that T3's reported latent state could not be reproduced.