Sepehr Hejazi

HTFAST enables high-throughput, quantitative affinity measurement of cell-free expressed binders using FRET, validated with nanobodies and SARS-CoV-2 sdAbs.

This paper introduces a cell-free combinatorial mutagenesis workflow using small oligos for high-iteration protein engineering, showing it is scalable and cost-effective.

This paper develops fluorescent single-walled carbon nanotube probes for measuring protease activity in cell-free expression reactions, enabling high-throughput screening and optimization.

This paper evaluates Oxford Nanopore sequencing for quantifying DNA heterogeneity in amplified DNA, finding Q5 and Phusion polymerases have the lowest error rates.