BiophysicsbioRxivPreprint
High-Level Expression, Purification and Biophysical Characterization of GPI-anchored native-like human Prion Protein using Leishmania tarentolae
Najoua Bolakhrif, Thomas A. Pauly, Luitgard Nagel‐Steger…·Lothar Gremer
This paper expresses and characterizes native-like human prion protein with GPI-anchoring and glycosylation using Leishmania tarentolae, confirming its structural homogeneity and monomeric conformation.
DOI 10.1101/2025.03.30.646161