Dieter Willbold

Ultra-Content Screening (UCS): Toward the Big Blood Picture

UCS enables high-throughput single-cell proteomic analysis of blood samples, revealing disease-specific expression profiles and immune subpopulations in acute myeloid leukemia.

Single-Stranded nucleic acid binding enhances the in vitro catalytic activity of Chikungunya virus nsP2 protease

This study identifies single-stranded nucleic acids that enhance the catalytic activity of Chikungunya virus nsP2 protease through specific interactions.

Minimal N-methylated and stapled peptide ligands for the autophagy protein GABARAP

This paper develops N-methylated and stapled peptide ligands for GABARAP with low nanomolar affinity and subfamily selectivity.

Inhibition of Chikungunya virus nsP2 protease in vitro by pantinin-1 isolated from scorpion venom

This study evaluates pantinin-1, a scorpion venom peptide, as an inhibitor of CHIKV nsP2 protease, showing effective inhibition with low micromolar affinity and no toxicity.

Accurate de novo design of high-affinity protein binding macrocycles using deep learning

This paper introduces RFpeptides, a deep learning framework for designing high-affinity macrocyclic peptides, achieving sub-nanomolar binding affinities and accurate structural predictions.

Lecanemab binds to transgenic mouse model-derived amyloid-β fibril structures resembling Alzheimer 's disease type-I, type-II and Arctic folds

This paper shows lecanemab binds to amyloid-β fibrils from Alzheimer's disease mouse models with flexible N-termini, including type-I, type-II, and Arctic folds, but not those with fixed N-termini like in tg-SwDI mice.

High-Level Expression, Purification and Biophysical Characterization of GPI-anchored native-like human Prion Protein using Leishmania tarentolae

This paper expresses and characterizes native-like human prion protein with GPI-anchoring and glycosylation using Leishmania tarentolae, confirming its structural homogeneity and monomeric conformation.

EGFR meets hATG8s - Biophysical and structural insights supporting a unique role of GABARAP during receptor trafficking

The paper identifies a GABARAP binding site on EGFR and shows its role in EGFR degradation through biophysical and structural analysis.

Direct disassembly of alpha-syn preformed fibrils into native alpha-syn monomers by an all-D-peptide

This paper develops all-D-peptide ligands that disassemble alpha-syn preformed fibrils into monomers, showing potential for treating Parkinson's disease.

Cryo-EM Structures of Amyloid-β Fibrils from Alzheimer's Disease Mouse Models

The paper presents cryo-EM structures of Aβ fibrils from Alzheimer's disease mouse models and compares them to human fibrils.

Conversion rate to the secondary conformation state in the binding mode of SARS-CoV-2 spike protein to human ACE2 may predict infectivity efficacy of the underlying virus mutant

The paper demonstrates that SARS-CoV-2 spike protein binding to ACE2 involves a two-step process with a secondary rate transition that enhances complex stability, and this is also observed in the B.1.1.7 variant.

Structural details of amyloid beta oligomers in complex with human prion protein as revealed by solid-state MAS NMR spectroscopy

This study uses solid-state MAS NMR to investigate the structure of Aβ oligomers bound to huPrP, revealing conformational heterogeneity and β-strand content.