High-Level Expression, Purification and Biophysical Characterization of GPI-anchored native-like human Prion Protein using Leishmania tarentolae
Najoua Bolakhrif, Thomas A. Pauly, Luitgard Nagel‐Steger, Dieter Willbold, Lothar Gremer
This paper expresses and characterizes native-like human prion protein with GPI-anchoring and glycosylation using Leishmania tarentolae, confirming its structural homogeneity and monomeric conformation.
bioRxiv · Mar 30, 2025 · PreprintBiophysics