Molecular BiologybioRxivPreprint
Revisiting the impact of synthetic ORF sequences on engineered LINE-1 retrotransposition
Dorothy Leung-Kwan Chan, Stephanie Workman, Nathan Smits…·Sandra R. Richardson
This study shows that synthetic mouse L1 retrotransposition efficiency is due to sequence changes and a 3UTR deletion, not just ORF GC content, and highlights the need to reconsider synthetic L1 constructs.
5 citations