Dorothy Leung-Kwan Chan

The paper investigates how L1 retrotransposition potential declines with age through methylation changes and promoter shortening in mice.

The paper identifies retrotransposon insertions as the main source of mutations in mouse induced pluripotent stem cells, showing hypomethylation of LINE-1 elements and their impact on gene regulation.

This study shows that synthetic mouse L1 retrotransposition efficiency is due to sequence changes and a 3UTR deletion, not just ORF GC content, and highlights the need to reconsider synthetic L1 constructs.