ImmunologyScience AdvancesPeer-reviewed
Deconvoluting TCR-dependent & -independent activation is vital for reliable Ag-specific CD4+ T cell characterization by AIM assay
Ming Z. M. Zheng, Lauren Burmas, Hyon‐Xhi Tan…·Jennifer A. Juno
The paper shows that AIM assays overestimate Ag-specific CD4+ T cells due to TCR-independent activation and introduces a modified assay using CXCR4-CD137+ cells for accurate characterization.
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