Author

Maria Sagan

ORCID 0000-0001-9190-4512Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:E. coli· 1 Approach:experimental assay· 1 Research Focus:protein folding & dynamics· 1 Assay:protein structure· 1

Frequent Co-Authors

Anna SzlachciciD· 1 shared Daria Gajewska· 1 shared Michał J. WalczakiD· 1 shared Paulina Krach· 1 shared

Activity by Year

2023-2023 · peak 1/yr

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4 top collaborators · 6 mutual co-author links

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1 shared paperBiochemistry

Anna SzlachcicORCID

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1 paper

Recent·Most cited

BiochemistrybioRxivPreprintDec 28, 2023

Expression Strategies for Recombinant HECT E3 Ligases in Escherichia coli

Paulina Krach, Daria Gajewska, Maria Sagan…·Michał J. Walczak

This study compares the expression of three HECT E3 ligases in E. coli, finding that lower temperatures improve solubility and yield, while full-length proteins are less successfully expressed than isolated domains.

DOI 10.1101/2023.12.28.573528

E. coliprotein structure

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