Chemically synthesized guide RNAs can direct CRISPR-CasRx cleavage of circRNAs with high efficiency and specificity
Karim Rahimi, Maria Schertz Andersen, Sabine Seeler, Thomas B. Hansen, Jørgen Kjems
This study shows that CRISPR-CasRx with synthetic guide RNAs efficiently and specifically depletes circRNAs with minimal off-target effects on linear RNAs.
bioRxiv · Aug 30, 2022 · PreprintMolecular Biology