Jørgen Kjems

This study uses single-particle tracking to compare early entry checkpoints of SARS-CoV-2 G614 and BA.5 variants, revealing variant-specific differences in binding and internalization.

This paper evaluates DNA-binding dyes for visualizing non-canonical extracellular nucleic acids in biofilms and identifies dyes with specific binding preferences.

This study investigates how recipient cell identity influences intracellular transport and membrane interactions of extracellular vesicles across species, finding that recipient-cell environment has a greater impact than EV origin.

The paper demonstrates the enzymatic synthesis of chemically modified RNA using a mutant T7 RNA polymerase and evaluates the impact of modifications on transcription efficiency and fidelity.

This paper introduces FY-RNA origami structures that are serum-stable and capable of molecular sensing and targeting, revealing structural insights through cryo-EM and simulations.

This paper introduces a method to efficiently synthesize LNA-modified RNAs using a mutant T7 RNA polymerase, demonstrating its utility in SELEX for generating therapeutically relevant aptamers with high affinity and nuclease resistance.

This study develops a mannosylated nucleic acid-based vaccine platform that targets Langerhans cells in the skin, enhancing antigen presentation and T cell activation.

This study evaluates C14120-based LNPs for mRNA delivery, showing correlations between lipid composition and transfection efficiency in vitro and organ-specific targeting in vivo.

This paper describes a DNA origami needle that targets cancer cells and delivers cargo into the cytosol by mimicking bacteriophage mechanisms.

The paper identifies surface-bound RNA on extracellular vesicles and shows it is co-delivered to target cells, suggesting a new RNA uptake mechanism.

The paper evaluates the CytoFLEX Nano flow cytometer's performance for analyzing extracellular vesicles in complex biofluids.

This study evaluates gel electrophoresis and HPLC-SEC for separating circRNAs, showing both rely on RNA denaturation and that magnesium ions impair separation.

The paper identifies a doxycycline-binding aptamer with exceptional regulatory properties in yeast and human cells, revealing a unique structural mechanism and validating its effectiveness through experimental methods.

This paper introduces a DNA origami stamping technique for precise nanoscale biomolecule patterning, validated using DNA-PAINT microscopy to assess efficiency and resolution.

This study develops a co-delivery system using cationic niosomes to deliver siRNA and doxorubicin, showing enhanced anticancer effects in MDA-MB-231 cells.

The paper demonstrates lipid nanoparticle-mediated mRNA delivery enables rapid, organ-wide protein expression in human-sized porcine donor kidneys during normothermic machine perfusion.

This study develops SPM-dots as a multifunctional carrier for miRNA delivery and stem cell tracking, showing enhanced osteogenic differentiation and in vivo labeling.

This study uses single-vesicle tracking to show α-synuclein oligomers form pores through a three-stage process influenced by membrane curvature, charge, lipids, and ligands.

This paper introduces a Snorkel-tag system for affinity purification of extracellular vesicles using CD81 fused with tags and transmembrane domains, enabling non-destructive isolation and future applications in tracing and analyzing EVs.

The paper shows that ciRS-7 and miR-7 regulate ischemia-induced neuronal death through glutamatergic signaling in mice.

This paper identifies circHIPK3 as a competing endogenous RNA that sequesters IGF2BP2 to regulate target mRNAs, including STAT3, and shows its correlation with bladder cancer survival.

The paper identifies brain-originating microRNAs in the blood of mice with experimental epilepsy, supporting their use as biomarkers.

This study identifies miR-335-5p as a regulator of voltage-gated sodium channels, showing that its modulation can influence seizure activity in epilepsy models.

This study shows that CRISPR-CasRx with synthetic guide RNAs efficiently and specifically depletes circRNAs with minimal off-target effects on linear RNAs.

The paper identifies differentially expressed circRNAs in AD brain regions and links their expression patterns to disease severity and pathways.

The paper identifies mammalian-wide interspersed repeats (MIRs) as facilitators of ciRS-7 circular RNA biosynthesis, revealing a new mechanism for circRNA formation.

This study uses RNA-seq to compare circRNA expression in lesional and non-lesional psoriasis skin, finding reduced circRNA abundance without clear links to miRNA binding site changes.

The paper presents a comprehensive human transcriptome atlas with single-nucleotide resolution, identifying thousands of novel RNA species and regulatory interactions.

The paper identifies functional miRNAs and their targets involved in seizure suppression in temporal lobe epilepsy using animal models and functional screens.