Tiziana Cesetti

The paper introduces 3D spheroid-based skin models and a deep-learning pipeline to study keratinocyte differentiation and barrier function, identifying NHK-E6/E7 as the best alternative to primary keratinocytes.

This study compares spheroid formation and behavior across six ultra-low attachment plate types using four human cell lines, finding size and eccentricity differences that suggest plate type impacts experimental outcomes.