Rüdiger Rudolf

Stage-resolved iPSC-to-motoneuron differentiation: Metabolic switch & mitochondrial remodeling

The paper examines metabolic changes during human iPSC differentiation into motoneurons, revealing a nonlinear metabolic transition rather than a simple switch.

Advanced 3D spheroid-based skin models and deep-learning based image analysis enable in-depth investigation of keratinocyte differentiation and barrier function

The paper introduces 3D spheroid-based skin models and a deep-learning pipeline to study keratinocyte differentiation and barrier function, identifying NHK-E6/E7 as the best alternative to primary keratinocytes.

From in vitro to in silico: a pipeline for generating virtual tissue simulations from real image data

The paper develops an accessible pipeline for generating virtual tissue simulations from real 3D image data using open-source software and the Cellular Potts Model.

Poly-alanine-tailing is a modifier of neurodegeneration caused by Listerin mutation

This study shows that impairing NEMF's poly-alanine-tailing activity in mice interacts with Listerin mutations to cause neurodegeneration, revealing a role for Ala-tailing in proteostasis and neurodegeneration.

A Multiparametric Analysis Reveals Differential Behavior of Spheroid Cultures on Distinct Ultra-Low Attachment Plates Types

This study compares spheroid formation and behavior across six ultra-low attachment plate types using four human cell lines, finding size and eccentricity differences that suggest plate type impacts experimental outcomes.