Advanced 3D spheroid-based skin models and deep-learning based image analysis enable in-depth investigation of keratinocyte differentiation and barrier function
Tiziana Cesetti, Claudia Buerger, Nathalie Couturier…·Rüdiger Rudolf
The paper introduces 3D spheroid-based skin models and a deep-learning pipeline to study keratinocyte differentiation and barrier function, identifying NHK-E6/E7 as the best alternative to primary keratinocytes.