Combinatorial pathway engineering using multiplex Serine recombinase-Assisted Genome Engineering (mSAGE)
Jay D. Huenemann, Austin L. Carroll, Joshua R. Elmore, Gara N. Dexter, Thomas D. Mand, Dawn M. Klingeman, William G. Alexander, Adam M. Guss
The paper introduces mSAGE, a method for multiplexed DNA insertion in Pseudomonas putida, and uses it to construct a combinatorial gene library for isophthalate degradation.
bioRxiv · Jul 11, 2025 · PreprintMicrobiology