William G. Alexander

The paper introduces mSAGE, a method for multiplexed DNA insertion in Pseudomonas putida, and uses it to construct a combinatorial gene library for isophthalate degradation.

The paper evaluates nearly 500,000 unique gRNAs in E. coli, finding most are functional and identifying rules to improve gRNA design.

The paper demonstrates that RNA thermometers can reduce Cas9-induced cell death by controlling its expression through temperature-dependent regulation.

The paper compares a reduced-genome Rhodopseudomonas strain to others, finding it retains core metabolic versatility but lacks genes for high-affinity transport and biodegradation.

This study uses experimental evolution to identify mutations that enhance catabolism of a lignin-derived aromatic dimer in Novosphingobium aromaticivorans, revealing a key enzyme involved in its degradation.

The paper introduces MIJAMP, a software tool to identify methylated DNA motifs from nanopore sequencing data, validated using genome-wide methylation data.

This study improves gene expression and transformation efficiency in Picosynechococcus sp. PCC 7002 by optimizing protocols and identifying a better integration site.

This paper develops a novel feature set and public resource for predicting CRISPR-Cas9 sgRNA efficiency using quantum chemical tensors and explainable-AI methods.

The paper constructs six-species synthetic yeast hybrids and shows they are chromosomally unstable but can rapidly adapt to novel environments.